Back to Search
Start Over
Strategies for the covalent conjugation of a bifunctional chelating agent to albumin: Synthesis and characterization of potential MRI contrast agents
- Source :
-
Journal of Inorganic Biochemistry . Feb2011, Vol. 105 Issue 2, p250-255. 6p. - Publication Year :
- 2011
-
Abstract
- Abstract: With the purpose to develop macromolecular magnetic resonance imaging contrast agents, we herein report three different synthetic approaches to the covalent attachment of bifunctional chelating agents to human serum albumin followed by coordination to contrast enhancing gadolinium(III). Applied methods cover active ester-mediated conjugation, linkage through glutaryl spacer, as well as the connection by the employment of glutaraldehyde. The content of gadolinium(III) was evaluated by inductively-coupled-plasma mass-spectrometry (ICP-MS) measurements and indicated reproducible amounts of conjugated contrast enhancing material. Small angle X-ray scattering (SAXS) experiments provided the size and altered shape of the gadolinium loaded proteins in comparison to unmodified albumin. Finally, the magnetic resonance properties of the protein conjugates were evaluated. The results indicated suitability of the gadolinium(III) loaded protein conjugates for use as macromolecular contrast agents in magnetic resonance imaging (MRI). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01620134
- Volume :
- 105
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Inorganic Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 57162341
- Full Text :
- https://doi.org/10.1016/j.jinorgbio.2010.10.015