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Electrochemical immunosensor of tumor necrosis factor α based on alkaline phosphatase functionalized nanospheres
- Source :
-
Biosensors & Bioelectronics . Jan2011, Vol. 26 Issue 5, p1890-1894. 5p. - Publication Year :
- 2011
-
Abstract
- Abstract: A novel immunosensor for sensitive detection of tumor necrosis factor α was reported. First of all, gold nanoparticles were uniformly assembled on the surface of poly (styrene-acrylic acid) nanospheres, which was used as the matrix to conjugate alkaline phosphatase (ALP). And then, the obtained composite was used as multi-enzyme functionalized label for immunoassay. Biocompatible polyaniline doped with poly (acrylic acid) was electro-polymerized at the glass carbon electrode to construct the matrix for the immobilization of antibody TNF-α. After the sandwich immunoreaction, the labeled ALP was used to hydrolyze α-naphthyl phosphate to produce the electroactive α-naphthol, which could be amperometrically detected. The results showed that the electrochemical signals were proportional to the logarithm of the antigen concentration in the range of 0.02–200.00ng/mL with the detection limit of 0.01ng/mL. The developed immunoassay showed high sensitivity, acceptable stability and reproducibility, which might have potentially broad applications in protein diagnostics and bioassay. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 26
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 57163295
- Full Text :
- https://doi.org/10.1016/j.bios.2010.03.025