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Combined VEGF and LMP-1 delivery enhances osteoprogenitor cell differentiation and ectopic bone formation.
- Source :
-
Growth Factors . Feb2011, Vol. 29 Issue 1, p36-48. 13p. - Publication Year :
- 2011
-
Abstract
- A novel strategy to enhance bone repair is to combine angiogenic factors and osteogenic factors. We combined vascular endothelial growth factor (VEGF) and LIM mineralization protein-1 (LMP-1) by using an internal ribosome entry site to link the genes within a single plasmid. We then evaluated the effects on osteoblastic differentiation in vitro and ectopic bone formation in vivo with a subcutaneously placed PLAGA scaffold loaded with a cloned mouse osteoprogenitor cell line, D1, transfected with plasmids containing VEGF and LMP-1 genes. The cells expressing both genes elevated mRNA expression of RunX2 and β-catenin and alkaline phosphatase activity compared to cells from other groups. In vivo, X-ray and micro-CT analysis of the retrieved implants revealed more ectopic bone formation at 2 and 3 weeks but not at 4 weeks compared to other groups. The results indicate that the combination of the therapeutic growth factors potentiates cell differentiation and may promote osteogenesis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08977194
- Volume :
- 29
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Growth Factors
- Publication Type :
- Academic Journal
- Accession number :
- 57290507
- Full Text :
- https://doi.org/10.3109/08977194.2010.544656