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Ultra-photostable, non-cytotoxic, and highly fluorescent quantum nanospheres for long-term, high-specificity cell imaging
- Source :
-
Biomaterials . Mar2011, Vol. 32 Issue 8, p2133-2140. 8p. - Publication Year :
- 2011
-
Abstract
- Abstract: A new class of fluorescent quantum nanospheres (QNs) is directly achieved in aqueous phase through a facile one-pot microwave irradiation (MWI) strategy. Multi-color QNs with maximum emission wavelengths ranging from 525 to 610 nm and PLQY of 30–60% are facilely prepared through this new MWI strategy. In addition to strong fluorescence, these QNs possess excellent photostability, preserving ∼90% of the original intensity after 70 min high-power UV irradiation (100 W Xeon lamp). In sharp contrast, the fluorescence of CdTe/CdS/ZnS core-shell-shell quantum dots (QDs), recognized as established fluorescent probes with robust photostability, decrease to ∼50% under the same conditions. Besides, cytotoxicity assessment demonstrates that the prepared QNs exhibit favorable cytocompatibility to K562 cells with high concentration (3 μmol) and long-time incubation (24 h). Furthermore, cellular imaging results demonstrate that the as-prepared QNs are remarkably efficacious for long-term and high-specificity immunofluorescent cellular labeling, and multi-color cell imaging. Our systematical investigation clearly shows that these high-performance QNs may serve as practical and powerful tools for various biological researches, such as in vivo and in vitro imaging. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01429612
- Volume :
- 32
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 57510962
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2010.11.034