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Single enzyme nanoparticle for biomimetic CO sequestration.
- Source :
-
Journal of Nanoparticle Research . Jan2011, Vol. 13 Issue 1, p263-271. 9p. - Publication Year :
- 2011
-
Abstract
- Nanoparticle technology is being increasingly used in environmental sciences. We prepared single enzyme nanoparticle (SEN) by modifying the surface of carbonic anhydrase (CA) with a thin layer of organic/inorganic hybrid polymer. SEN-CA appears to be improving the stability of free enzyme. CA, as ubiquitously found enzyme, is involved in gaseous CO sequestration and is being looked as a promising candidate for combating global warming. We report here physical characterization of SEN-CA using transmission electron microscope (TEM), Fourier-transform infrared analysis (FTIR), X-ray diffraction analysis (XRD), and energy dispersive X-ray (EDX). Average size of SEN-CA particles appears to be in the range of 70-80 nm. We also report the effect of SEN formation on the kinetic parameters of free CA such as Michaelis-Menten constant ( K), maximum reaction velocity ( V), and storage stability of free CA and SEN-CA. The V of SEN-CA (0.02857 mmol/min/mg) and free enzyme (0.02029 mmol/min/mg) is almost similar. K has decreased from 6.143 mM for SEN-CA to 1.252 mM for free CA. The stabilization of CA by SEN formation results in improved the half-life period (up to 100 days). The formation of carbonate was substantiated by using gas chromatography (GC). The conversion of CO to carbonate was 61 mg of CaCO/mg of CA and 20.8 mg of CaCO/mg of CA using SEN-CA and free CA, respectively. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13880764
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Nanoparticle Research
- Publication Type :
- Academic Journal
- Accession number :
- 57639775
- Full Text :
- https://doi.org/10.1007/s11051-010-0026-z