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RNAi screening for fat regulatory genes with SRS microscopy.
- Source :
-
Nature Methods . Feb2011, Vol. 8 Issue 2, p135-138. 4p. 1 Diagram, 2 Graphs. - Publication Year :
- 2011
-
Abstract
- Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15487091
- Volume :
- 8
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Nature Methods
- Publication Type :
- Academic Journal
- Accession number :
- 57690253
- Full Text :
- https://doi.org/10.1038/nmeth.1556