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Accumulation and Separation of Membrane-Bound Proteins Using Hydrodynamic Forces.

Authors :
Jönsson, Peter
Gunnarsson, Anders
HöÔk, Fredrik
Source :
Analytical Chemistry. 1/15/2011, Vol. 83 Issue 2, p604-611. 8p.
Publication Year :
2011

Abstract

The separation of molecules residing in the cell membrane remains a largely unsolved problem in the fields of bioscience and biotechnolo. We demonstrate how hydrodynamic forces can be used to both accumulate and separate membrane-bound proteins in their native state. A supported lipid bilayer (SIB) was formed inside a microfluidic channel with the two proteins streptavidin (SA) and cholera toxin (Cl') coupled to receptors in the lipid bilayer The anchored proteins were first driven toward the edge of the lipid bilayer by hydrodynainic forces from a flowing liquid above the SIB, resulting in the accumulation of protein molecules at the edge of the bilayer. After the concentration process, the bulk flow of liquid in the channel was reversed and the accumulated proteins were driven away from the edge of the bilayer. Each type of protein was found to move at a characteristic drift velocity, determined by the frictional coupling between the protein and the lipid bilayer, as well as the size and shape of the protein molecule. Despite having a similar molecular weight, SA and CF could be separated into monomolecular populations using this approach. The method also revealed heterogeneity among the CF molecules, resulting in three subpopulations with different drift velocities. This was tentatively attributed to multivalent interactions between the protein and the monosialoganglioside GML receptors in the lipid bilayer. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032700
Volume :
83
Issue :
2
Database :
Academic Search Index
Journal :
Analytical Chemistry
Publication Type :
Academic Journal
Accession number :
57754354
Full Text :
https://doi.org/10.1021/ac102979b