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Molecular cloning, in vitro expression and bioactivity of rabbit transforming growth factor-beta receptor type II (rTGF-βRII)
- Source :
-
Veterinary Immunology & Immunopathology . Mar2011, Vol. 140 Issue 1/2, p55-62. 8p. - Publication Year :
- 2011
-
Abstract
- Abstract: Transforming growth factor-beta receptor II (TGF-βRII) is an attractive target for anti-scarring therapy in wound healing because it attenuates excessive TGF-β which has pleiotropic effects on the immune system. In the present study, the cDNA of rabbit TGF-βRII (rTGF-βRII) was amplified from rabbit peripheral blood by RT-PCR. The open reading frame of rTGF-βRII encodes a protein consisting of 567 amino acids, which contains a predicted transmembrane domain and a Serine/Threonine protein kinase domain similar to other identified mammalian TGF-βRIIs. The amino acid sequences of the biologically active, soluble rTGF-βRII and mouse, rat, human and chicken counterparts are 81%, 81%, 89% and 61%, respectively, identical. Recombinant soluble rTGF-βRII (rsTGF-βRII) fused with His tag was efficiently expressed in Escherichia coli BL21 (DE3) expression host strain. This fusion protein''s molecular weight of ∼19kDa was identified by SDS-PAGE and Western blotting. In vitro, purified rsTGF-βRII was able to inhibit the proliferation of keloid rabbit fibroblasts and decrease the level of collagen. These findings indicate that rTGF-βRII plays an important role in inhibiting the proliferation of keloid rabbit fibroblasts and provides the basis for investigations on the role of TGF-βRII in this important domestic species. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01652427
- Volume :
- 140
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Veterinary Immunology & Immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 57857908
- Full Text :
- https://doi.org/10.1016/j.vetimm.2010.11.014