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Effect of nitrogen-rich cell culture surfaces on type X collagen expression by bovine growth plate chondrocytes.
- Source :
-
BioMedical Engineering OnLine . 2011, Vol. 10 Issue 1, p1-12. 12p. 1 Color Photograph, 1 Black and White Photograph, 2 Charts, 6 Graphs. - Publication Year :
- 2011
-
Abstract
- Background: Recent evidence indicates that osteoarthritis (OA) may be a systemic disease since mesenchymal stem cells (MSCs) from OA patients express type X collagen, a marker of late stage chondrocyte hypertrophy (associated with endochondral ossification). We recently showed that the expression of type X collagen was suppressed when MSCs from OA patients were cultured on nitrogen (N)-rich plasma polymer layers, which we call "PPE:N" (N-doped plasma-polymerized ethylene, containing up to 36 atomic percentage (at.% ) of N. Methods: In the present study, we examined the expression of type X collagen in fetal bovine growth plate chondrocytes (containing hypertrophic chondrocytes) cultured on PPE:N. We also studied the effect of PPE:N on the expression of matrix molecules such as type II collagen and aggrecan, as well as on proteases (matrix metalloproteinase-13 (MMP-13) and molecules implicated in cell division (cyclin B2). Two other culture surfaces, "hydrophilic" polystyrene (PS, regular culture dishes) and nitrogen-containing cation polystyrene (Primaria®), were also investigated for comparison. Results: Results showed that type X collagen mRNA levels were suppressed when cultured for 4 days on PPE:N, suggesting that type X collagen is regulated similarly in hypertrophic chondrocytes and in human MSCs from OA patients. However, the levels of type X collagen mRNA almost returned to control value after 20 days in culture on these surfaces. Culture on the various surfaces had no significant effects on type II collagen, aggrecan, MMP-13, and cyclin B2 mRNA levels. Conclusion: Hypertrophy is diminished by culturing growth plate chondrocytes on nitrogen-rich surfaces, a mechanism that is beneficial for MSC chondrogenesis. Furthermore, one major advantage of such "intelligent surfaces" over recombinant growth factors for tissue engineering and cartilage repair is potentially large cost-saving. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1475925X
- Volume :
- 10
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BioMedical Engineering OnLine
- Publication Type :
- Academic Journal
- Accession number :
- 57996626
- Full Text :
- https://doi.org/10.1186/1475-925X-10-4