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Adipose tissue-derived stem cells show both immunogenic and immunosuppressive properties after chondrogenic differentiation.
- Source :
-
Cytotherapy (Taylor & Francis Ltd) . Mar2011, Vol. 13 Issue 3, p310-317. 8p. 1 Color Photograph, 1 Black and White Photograph, 2 Charts, 1 Graph. - Publication Year :
- 2011
-
Abstract
- Background aims. The chondrogenic differentiation potential of mesenchymal stromal cells (MSC), as well as their immunosuppressive properties, have been studied extensively. So far, only a few studies have addressed the question of whether MSC still retain their immunosuppressive qualities after transdifferentiation. In particular, the expression of immunogenic markers, such as human leukocyte antigen (HLA)-DR, after differentiation has never been investigated. Methods. Chondrogenic transdifferentiation was induced in human adipose tissue-derived stem cell (ADSC) pellet cultures derived from 10 different patients, using 10 ng/mL transforming growth factor (TGF)-ββ3. Samples were harvested over a time--course of 28 days and analyzed by immunohistochemistry and reverse transcription (RT)--polymerase chain reaction (PCR). The cytokine levels in the supernatants of the samples were measured semi-quantitatively by dot-blots and quantitatively by enzyme-linked immunosorbant assays (ELISA). Results. Undifferentiated ADSC were negative for chondrogenic markers, as well as HLA-ABC and HLA-DR epitopes in immunofluorescence. In contrast, TGF-ββ3-induced pellet cultures showed both expression of chondrogenic differentiation markers, such as transcription factor 9 (Sox 9), collagen type IIa and aggrecan, and an up-regulation of HLA-DR, beginning at day 7 after induction. Interferon-γγ (INF-γγ) is known to up-regulate HLA-DR. Therefore we measured INF-γγ levels in the supernatants of TGF-ββ3-induced pellets and, indeed, INF-γγ was up-regulated during chondrogenesis in ADSC pellet cultures. However, both undifferentiated and TGF-ββ3-induced ADSC also showed expression of immunosuppressive HLA-G and interleukin (IL)-10 up-regulation. Conclusions. These results suggest that the immunogenicity of adult stem cell-derived tissue should be tested in animal models before clinical trials for allogeneic engineered tissue are considered. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14653249
- Volume :
- 13
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Cytotherapy (Taylor & Francis Ltd)
- Publication Type :
- Academic Journal
- Accession number :
- 58059405
- Full Text :
- https://doi.org/10.3109/14653249.2010.504769