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Purification of CD4+ T Cells for Adoptive Immunotherapy after Allogeneic Hematopoietic Stem Cell Transplantation
- Source :
-
Biology of Blood & Marrow Transplantation . Mar2011, Vol. 17 Issue 3, p374-383. 10p. - Publication Year :
- 2011
-
Abstract
- Donor lymphocyte infusions (DLIs) are used for adoptive immunotherapy to prevent or treat relapse and infectious complications after allogeneic hematopoietic stem cell transplantation (HSCT). Unmanipulated DLIs are associated with a risk of graft-versus-host disease (GVHD), probably related to CD8+ T cell activity. We investigated an automated clinical-scale human-CD4+-cell purification method to deplete CD8+ cells. Twenty-four stem cell recipients received a total of 24 leukapheresis products being enriched for CD4+ cells using magnetic associated cell sorting (MACS) with an automated device (CliniMACS®) before DLIs. MACS resulted in a mean CD4+ cell count of 16 × 106/kg bw corresponding to 3.4-fold CD4+ cell enrichment. Mean yield and purity of CD45+CD3+CD4+CD14−7AAD− were 74% ± 23% and 82% ± 11%, respectively. Median initial dose of DLIs was 1.1 × 106 CD4+/kg. During a median follow-up of 25 months, 7 (30%) patients experienced GVHD (acute II-IV: n = 4, 17%; acute III-IV: n = 2, 8%; chronic limited: n = 2, 8%; chronic extensive: n = 1, 4%). Thirteen of 21 further evaluable patients (62%) showed measurable clinical response, 2 patients with therapy refractory infectious complications (HSV) showed remarkable immunologic improvement. Automated enrichment of CD4+ by magnetic cell sorting provides an efficient and rapid method for processing donor lymphocytes. Additional studies should further investigate this approach in terms of efficacy and the risk of GVHD. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 10838791
- Volume :
- 17
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biology of Blood & Marrow Transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 58098822
- Full Text :
- https://doi.org/10.1016/j.bbmt.2010.07.003