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Cloning and characterization of a delta-6 desaturase encoding gene from Nannochloropsis oculata.
- Source :
-
Chinese Journal of Oceanology & Limnology . Mar2011, Vol. 29 Issue 2, p290-296. 7p. - Publication Year :
- 2011
-
Abstract
- gene ( NANOC-D6D) encoding a desaturase that removes two hydrogen atoms from fatty acids at delta 6 position was isolated from a cDNA library of Nannochloropsis oculata (Droop) D. J. Hibberd (Eustigmatophyceae). The unicellular marine microalga N. oculata synthesizes rich long chain polyunsaturated fatty acids (LCPUFAs), including eicosapentaenoic acid (20:5n-3, EPA). The deduced protein contains 474 amino acids that fold into 4 trans-membrane domains. The neighbor-joining phylogenetic tree indicates that NANOC-D6D is phylogenetically close to the delta-6 fatty acid desaturase of marine microalgae such as Glossomastix chrysoplasta, Thalassiosira pseudonana, and Phaeodactylum tricornutum. The gene was expressed in Saccharomyces cerevisiae INVScl to verify the substrate specificity of NANOC-D6D. Our results suggest that the recombinant NANOC-D6D simultaneously desaturates linoleic acid (LA) and α-linolenic acid (ALA). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02544059
- Volume :
- 29
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Chinese Journal of Oceanology & Limnology
- Publication Type :
- Academic Journal
- Accession number :
- 58721512
- Full Text :
- https://doi.org/10.1007/s00343-011-0048-0