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Immunochemical studies of antigenic lymphoma-associated proteins.
- Source :
-
British Journal of Haematology . Jan2002, Vol. 116 Issue 1, p135-141. 7p. - Publication Year :
- 2002
-
Abstract
- Summary. We have previously reported that plasma from patients with anaplastic lymphoma kinase (ALK)-positive lymphoma contains antibodies against the oncogenic kinase NPM-ALK protein characteristic of this disease. We investigated whether this reactivity represents a phenomenon unique to ALK-positive lymphoma by screening plasma from patients with follicular lymphoma for antibodies to BCL-2 protein. Eight out of 10 samples showed such reactivity (and in six cases gave specific staining of BCL-2-transfected cells). As these findings suggest a new biochemical approach to the identification of oncogenic proteins in lymphoma, we investigated whether antibodies present in patients with ALK-positive lymphoma can precipitate NPM-ALK in quantities which should be sufficient for further analysis. We found that plasma samples from all10 patients studied immunoprecipitated NPM-ALK asaprotein visible in silver-stained sodium dodecyl sulphatepolyacrylamide gels. Finally we demonstrated that NPM-ALK could be visualized more clearly if it were immunoprecipitated from extracts of cells in which newly synthesized proteins had been labelled with 35 S and then identified by autoradiography. These results suggest a strategy for using patients' autoantibodies to screen for antibodies to other tumour-associated proteins. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LYMPHOMAS
*PROTEIN kinases
*ONCOGENES
*IMMUNOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 00071048
- Volume :
- 116
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- British Journal of Haematology
- Publication Type :
- Academic Journal
- Accession number :
- 5893235
- Full Text :
- https://doi.org/10.1046/j.1365-2141.2002.03252.x