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Cloning and characterization of a hsp70 gene from Asiatic hard clam Meretrix meretrix which is involved in the immune response against bacterial infection

Authors :
Yue, Xin
Liu, Baozhong
Sun, Li
Tang, Baojun
Source :
Fish & Shellfish Immunology. Mar2011, Vol. 30 Issue 3, p791-799. 9p.
Publication Year :
2011

Abstract

Abstract: In the present study, a 71.43kDa heat shock protein cDNA was cloned from Asiatic hard clam Meretrix meretrix. The cDNA was 2292bp, containing an open reading frame (ORF) of 1959bp, which encodes a protein of 652 amino acids with a theoretical molecular weight of 71.43kDa and an isoelectric point of 5.32. Based on the amino acid sequence analysis and phylogenetic analysis, this hsp70 cDNA is a member of cytoplasmic hsc70 (constitutive genes) subfamily in the hsp70 family, and is designated as MmeHsc71. Quantitative RT-PCR was carried out to compare the spatial and temporal expression patterns of MmeHsc71 in the mRNA level between control clams and Vibrio parahaemolyticus-infected clams. Spatially, MmeHsc71 mRNA was found in all tested tissues, including foot, hepatopancreas, mantle and gill. MmeHsc71 mRNA expression level in hepatopancreas and gill displayed a significant increase in vibrio-challenged clams at 24h post-infection compared to control clams (P <0.05). Temporally, there was a significant increase of MmeHsc71 mRNA level in hepathopancreas of vibrio-challenged clams compared to control clams at 6, 12, and 24h post-challenge, respectively. The result of quantitative immunofluorescence also indicated that there was obvious increase of MmeHsc71 in hepatopancreas of vibrio-challenged clams compared to control clams in protein level at 24h post-infection. The results suggested that MmeHsc71 may play an important role in mediating the immune responses of M. meretrix to bacterial challenge. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
10504648
Volume :
30
Issue :
3
Database :
Academic Search Index
Journal :
Fish & Shellfish Immunology
Publication Type :
Academic Journal
Accession number :
59166962
Full Text :
https://doi.org/10.1016/j.fsi.2010.12.027