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Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae.
- Source :
-
Journal of General Plant Pathology . May2011, Vol. 77 Issue 3, p147-151. 5p. 1 Diagram, 2 Graphs. - Publication Year :
- 2011
-
Abstract
- Here we report a novel system to induce RNA silencing in Magnaporthe oryzae using the retrotransposon MAGGY. The gene-silencing vector pSilent-MG carries a MAGGY variant with a unique cloning site in the 3′ UTR region and a geneticin-resistance cassette as a selection marker. A gene-silencing assay with a GFP gene revealed that pSilent-MG vectors induced gene silencing at a practical level. In the resulting GFP-silenced transformants, GFP siRNAs accumulated and DNA methylation increased in the GFP-expressing gene, implying that the vector could induce both transcriptional and post-transcriptional gene silencing. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PYRICULARIA oryzae
*TRANSPOSONS
*GENE silencing
*RNA
*BIOMARKERS
Subjects
Details
- Language :
- English
- ISSN :
- 13452630
- Volume :
- 77
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of General Plant Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 60173817
- Full Text :
- https://doi.org/10.1007/s10327-011-0302-5