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Genome-wide analysis of 5-hydroxymethylcytosine distribution reveals its dual function in transcriptional regulation in mouse embryonic stem cells.
- Source :
-
Genes & Development . 4/1/2011, Vol. 25 Issue 7, p3-3. 1p. - Publication Year :
- 2011
-
Abstract
- Recent studies have demonstrated that the Ten-eleven translocation (Tet) family proteins can enzymatically convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). While 5mC has been studied extensively, little is known about the distribution and function of 5hmC. Here we present a genome-wide profile of 5hmC in mouse embryonic stem (ES) cells. A combined analysis of global 5hmC distribution and gene expression profile in wild-type and Tet1-depleted ES cells suggests that 5hmC is enriched at both gene bodies of actively transcribed genes and extended promoter regions of Polycomb-repressed developmental regulators. Thus, our study reveals the first genome-wide 5hmC distribution in pluripotent stem cells, and supports its dual function in regulating gene expression. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEINS
*EMBRYONIC stem cells
*GENOMES
*MICE
*CHROMOSOMAL translocation
Subjects
Details
- Language :
- English
- ISSN :
- 08909369
- Volume :
- 25
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Genes & Development
- Publication Type :
- Academic Journal
- Accession number :
- 60189354
- Full Text :
- https://doi.org/10.1101/gad.2036011