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Effect of Film Thickness on the Antifouling Performance of Poly(hydroxy-functional methacrylates) Grafted Surfaces.
- Source :
-
Langmuir . Apr2011, Vol. 27 Issue 8, p4906-4913. 8p. - Publication Year :
- 2011
-
Abstract
- The development of nonfouling biomaterials to prevent nonspecific protein adsorption and cell/bacterial adhesion is critical for many biomedical applications, such as antithrombogenic implants and biosensors. In this work, we polymerize two types of hydroxy-functional methacrylates monomers of 2-hydroxyethyl methacrylate (HEMA) and hydroxypropyl methacrylate (HPMA) into polymer brushes on the gold substrate via surface-initiated atom transfer radical polymerization (SI-ATRP). We systematically examine the effect of the film thickness of polyHEMA and polyHPMA brushes on their antifouling performance in a wide range of biological media including single-protein solution, both diluted and undiluted human blood serum and plasma, and bacteria culture. Surface plasmon resonance (SPR) results show a strong correlation between antifouling property and film thickness. Too thin or too thick polymer brushes lead to large protein adsorption. Surfaces with the appropriate film thickness of â¼25â45 nm for polyHPMA and â¼20â45 nm for polyHEMA can achieve almost zero protein adsorption (<0.3 ng/cm2) from single-protein solution and diluted human blood plasma and serum. For undiluted human blood serum and plasma, polyHEMA brushes at a film thickness of â¼20â30 nm adsorb only â¼3.0 and â¼3.5 ng/cm2proteins, respectively, while polyHPMA brushes at a film thickness of â¼30 nm adsorb more proteins of â¼13.5 and â¼50.0 ng/cm2, respectively. Moreover, both polyHEMA and polyHPMA brushes with optimal film thickness exhibit very low bacteria adhesion. The excellent antifouling ability and long-term stability of polyHEMA and polyHPMA brushes make them, especially for polyHEMA, effective and stable antifouling materials for usage in blood-contacting devices. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07437463
- Volume :
- 27
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Langmuir
- Publication Type :
- Academic Journal
- Accession number :
- 60313112
- Full Text :
- https://doi.org/10.1021/la200061h