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Coordinated age-dependent and pancreatic-specific expression of mouse Reg2 Reg3αα, and Reg3ββ genes.
- Source :
-
Growth Factors . Apr-Jun2011, Vol. 29 Issue 2/3, p72-81. 10p. 2 Color Photographs, 1 Chart, 3 Graphs. - Publication Year :
- 2011
-
Abstract
- Reg family proteins such as Reg1 and islet neogenesis-associated protein (INGAP) have long been implicated in the growth and/or neogenesis of pancreatic islet cells. Recent reports further suggest similar roles to be played by new members such as Reg2, Reg3αα, and Reg3ββ. We have studied their age-, isoform-, and tissue-specific expressions. RNA and protein were isolated from C57BL/6 mice aged 7, 30, and 90 days. Using real-time polymerase chain reaction, the levels of Reg gene expression in the pancreas were 20--600-fold higher than that in other tissues (≫≫duodenum>stomach>liver); gene expression of Reg2, Reg3αα, and Reg3ββ was age dependent as it was hardly detectable at day 7, increased drastically at day 30, and significantly decreased at day 90; the levels of pancreatic proteins displayed similar age-dependent variations. Using dual-labeled immunofluorescence, Reg2, Reg3αα, and Reg3ββ were abundantly expressed in most acinar cells of the pancreas, in contrast to INGAP which exhibited stepwise increases from day 7 to day 90 and colocalized with the αα-cells. These new Reg genes were mainly expressed in the pancreas, with clear age-dependent and isoform-specific patterns. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08977194
- Volume :
- 29
- Issue :
- 2/3
- Database :
- Academic Search Index
- Journal :
- Growth Factors
- Publication Type :
- Academic Journal
- Accession number :
- 60466765
- Full Text :
- https://doi.org/10.3109/08977194.2011.562866