Retrospective Application of Transposon-Directed Insertion Site Sequencing to a Library of Signature-Tagged Mini-Tn5Km2 Mutants of Escherichia coli O157:H7 Screened in Cattle.

Authors :: Eckert, Sabine E.
Dziva, Francis
Chaudhuri, Roy R.
Langride, Gemma C.
Turner, Daniel J.
Pickard, Derek J.
Maskell, Duncan J.
Thompson, Nicholas R.
Stevens, Mark P.
Source :: Journal of Bacteriology. Apr2011, Vol. 193 Issue 7, p1771-1776. 6p. 2 Diagrams, 3 Charts, 2 Graphs.
Publication Year :: 2011
Abstract: Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% ofEscherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coil O157:H7 genome without further animal use. [ABSTRACT FROM AUTHOR]

Subjects :: *TRANSPOSONS
*ESCHERICHIA coli O157:H7
*MUTAGENESIS
*GENOMES
*GENETIC mutation
*CATTLE

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