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The sequence selectivity of DNA-targeted 9-aminoacridine cisplatin analogues in a telomere-containing DNA sequence.
- Source :
-
Journal of Biological Inorganic Chemistry (JBIC) . May2011, Vol. 16 Issue 5, p735-743. 9p. - Publication Year :
- 2011
-
Abstract
- In this study, the detailed DNA sequence specificity of four acridine Pt complexes was examined and compared with that of cisplatin. The DNA sequence specificity was determined in a telomere-containing DNA sequence using a polymerase stop assay, with a fluorescent primer and an automated capillary DNA sequencer. The Pt compounds included an acridine intercalating moiety that was modified to give a 9-aminoacridine derivative, a 7-methoxy-9-aminoacridine derivative, a 7-fluoro-9-aminoacridine derivative and a 9-ethanolamine-acridine derivative. Compared with cisplatin, the DNA sequence specificity was most altered for the 7-methoxy-9-aminoacridine compound, followed by the 9-aminoacridine derivative, the 7-fluoro-9-aminoacridine compound and the 9-ethanolamine-acridine derivative. The DNA sequence selectivity for the four acridine Pt complexes was shifted away from runs of consecutive guanines towards single guanine bases, especially 5′-GA dinucleotides and sequences that contained 5′-CG. The sequence specificity was examined in telomeric and non-telomeric DNA sequences. Although it was found that telomeric DNA sequences were extensively damaged by the four acridine Pt complexes, there was no extra preference for telomeric sequences. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09498257
- Volume :
- 16
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Inorganic Chemistry (JBIC)
- Publication Type :
- Academic Journal
- Accession number :
- 60672645
- Full Text :
- https://doi.org/10.1007/s00775-011-0774-y