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A fast solution switching system with temperature control for single cell measurements
- Source :
-
Journal of Neuroscience Methods . Jul2011, Vol. 199 Issue 1, p35-42. 8p. - Publication Year :
- 2011
-
Abstract
- Abstract: This article describes a perfusion system for biophysical single cell experiments at the physiological temperature. Our system regulates temperature of test solutions using a small heat exchanger that includes several capillaries. Water circulating inside the heat exchanger warms or cools test solutions flowing inside the capillaries. Temperature-controlled solutions are delivered directly to a single cell(s) through a multibarreled manifold that switches solutions bathing a cell in less than 1s. This solution exchange is optimal for patch clamp, single-cell microamperometry, and microfluorometry experiments. Using this system, we demonstrate that exocytosis from pancreatic β cells and activation of TRPV1 channels are temperature sensitive. We also discuss how to measure local temperature near a single cell under investigation. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01650270
- Volume :
- 199
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Neuroscience Methods
- Publication Type :
- Academic Journal
- Accession number :
- 61237443
- Full Text :
- https://doi.org/10.1016/j.jneumeth.2011.04.023