DIRECT IMPACT CORONA IONIZATION OF BACTERIA FOR RAPID, REPRODUCIBLE IDENTIFICATION VIA SPECTRAL PATTERN RECOGNITION.

A novel atmospheric pressure ionization process, Direct Impact Corona Ionization (DICI), is described here. In this process, a corona impinges onto the flat surface of a stainless steel pin carrying a thin film of dried bacterial suspension, the analyte. Two electrodes-a corona electrode and the sample pin-are immersed in hot inert He gas flux, flowing past them towards a 0.4 mm orifice leading to a mass spectrometer analyzer. An electric potential of 1.5-3.0 kV is placed between the two. At distances less than 1 cm, an intermittent arc is formed. At approximately 4 mm, the arc becomes a continuous corona discharge (plasma). The plasma is hot enough to: A) locally melt the impact zone on the steel pin, and B) ablate the dry thin bacterial film deposited on the metal pin. Biomolecular ions as heavy as 790 m/z are generated. Mass spectral fingerprints of bacteria are obtained with a high degree of reproducibility by selecting the highest intensity of an 'indicator ion', 560.5 m/z or another relatively heavy ion whose appearance signals efficient vaporization of low volatility components. [ABSTRACT FROM AUTHOR]