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In vivo cellular MRI of dendritic cell migration using micrometer-sized iron oxide (MPIO) particles.
- Source :
-
Molecular Imaging & Biology . Aug2011, Vol. 13 Issue 4, p679-694. 16p. - Publication Year :
- 2011
-
Abstract
- <bold>Purpose: </bold>This study seeks to assess the use of labeling with micron-sized iron oxide (MPIO) particles for the detection and quantification of the migration of dendritic cells (DCs) using cellular magnetic resonance imaging (MRI).<bold>Procedures: </bold>DCs were labeled with red fluorescent MPIO particles for detection by cellular MRI and a green fluorescent membrane dye (PKH67) for histological detection. MPIO-labeled DCs or unlabeled control DCs were injected into mice footpads at two doses (0.1 × 10(6) and 1 × 10(6)). Images were acquired at 3 Tesla before DC injection and 2, 3, and 7 days post-DC injection.<bold>Results: </bold>Labeling DCs with MPIO particles did not affect viability, but it did alter markers of DC activation and maturation. MRI and fluorescence microscopy allowed for the detection of MPIO-labeled DCs within the draining popliteal nodes after their injection into the footpad.<bold>Conclusions: </bold>This paper presents the first report of the successful use of fluorescent MPIO particles to label and track DC migration. [ABSTRACT FROM AUTHOR]
- Subjects :
- *IRON oxides
*DENDRITIC cells
*MAGNETIC resonance imaging
*HISTOLOGY
*CELLS
*FLUORESCENCE microscopy
*ANIMAL experimentation
*APOPTOSIS
*BONE marrow
*CELL physiology
*CELL motility
*COMPARATIVE studies
*IRON compounds
*LIGHT
*LYMPH nodes
*RESEARCH methodology
*MEDICAL cooperation
*MICE
*ORGANIC compounds
*PARTICLES
*RESEARCH
*STAINS & staining (Microscopy)
*PHENOTYPES
*EVALUATION research
Subjects
Details
- Language :
- English
- ISSN :
- 15361632
- Volume :
- 13
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Molecular Imaging & Biology
- Publication Type :
- Academic Journal
- Accession number :
- 62001517
- Full Text :
- https://doi.org/10.1007/s11307-010-0403-0