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A Microbial Glycolipid Functions as a New Class of Target Antigen for Delayed-type Hypersensitivity.
- Source :
-
Journal of Biological Chemistry . 5/13/2011, Vol. 286 Issue 19, p16800-16806. 7p. - Publication Year :
- 2011
-
Abstract
- Delayed-type hypersensitivity (DTH) is marked by high levels of protein antigen-specific T cell responses in sensitized individuals. Recent evidence has revealed a distinct pathway for T cell immunity directed against glycolipid antigens, but DTH to this class of antigen has been undetermined and difficult to prove due to their insolubility in aqueous solutions. Here, glucose monomycolate (GMM), a highly hydrophobic glycolipid of the cell wall of mycobacteria, was dispersed in aqueous solutions in the form of octaarginine-modified liposomes and tested for its ability to elicit cutaneous DTH responses in bacillus Calmette-Guerin (BCG)-immunized guinea pigs. After an intra-dermal challenge with the GMM liposome, a significant skin induration was observed in BCG-immunized, but not mock-treated, animals. The skin reaction peaked at around 2 days with local infiltration by mononuclear cells, and therefore, the response shared basic features with the classical DTH to protein antigens. Lymph node T cells from BCG-immunized guinea pigs specifically increased IFN-γ transcription in response to the GMM liposome, and this response was completely blocked by antibodies to CD1 lipid antigen-presenting molecules. Finally, whereas the T cells increased transcription of both T helper (Th) 1-type (IFN-γ and TNF-α) and Th2-type (IL-5 and IL-10) cytokines in response to the purified protein derivative or tuberculin, their GMM-specific response was skewed to Th1-type cytokine production known to be critical for protection against tuberculosis. Thus, our study reveals a novel form of DTH with medical implications. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DELAYED hypersensitivity
*BCG vaccines
*T cells
*TUBERCULIN
*IMMUNOREGULATION
Subjects
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 286
- Issue :
- 19
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 62251121
- Full Text :
- https://doi.org/10.1074/jbc.M110.217224