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Inhibitory effects of GL-V9 on the invasion of human breast carcinoma cells by downregulating the expression and activity of matrix metalloproteinase-2/9
- Source :
-
European Journal of Pharmaceutical Sciences . Aug2011, Vol. 43 Issue 5, p393-399. 7p. - Publication Year :
- 2011
-
Abstract
- Abstract: Cancer cell invasion plays a crucial role in growth and local spreading of tumors. GL-V9 is a newly synthesized flavonoid that has been shown to possess an antitumor effect. However, the mechanism of GL-V9 in preventing tumor growth is still unclear. The purpose of this study is to investigate the anti-invasive and anti-metastatic activity of this novel compound in MDA-MB-231 and MCF-7 human breast carcinoma cells. In this study, GL-V9 caused a concentration-dependent suppression of cell adhesive ability by cell adhesion assay, it also inhibited the migration and invasion of cells by wound healing assay and transwell invasion assay in a concentration-dependent manner. Considering matrix metalloproteinases (MMPs) play an important role in metastatic process, we used western blotting and gelatin zymography to examine the effect of GL-V9 on the expression and activity of MMPs. The mechanism revealed that GL-V9 significantly suppressed the expression and activity of MMP-2 and MMP-9. Furthermore, GL-V9 suppressed their upstream protein kinases activation by reducing phosphorylated forms of serine/threonine kinase AKT and c-Jun N-terminal kinase. These findings suggested that GL-V9 could inhibit the invasion of tumor cells by downregulating the expression and activity of MMP-2 and MMP-9, potentially associating with the suppression of phosphorylation of AKT and JNK. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09280987
- Volume :
- 43
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- European Journal of Pharmaceutical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 62270260
- Full Text :
- https://doi.org/10.1016/j.ejps.2011.06.001