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Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18
- Source :
-
Bioresource Technology . Sep2011, Vol. 102 Issue 17, p8052-8056. 5p. - Publication Year :
- 2011
-
Abstract
- Abstract: Pseudomonas fluorescens BIT-18 was isolated from soil near a vegetable oil factory and shown to produce a B-type phospholipase. The enzyme was partially purified by ammonium sulfate precipitation. Gas chromatography demonstrated that the enzyme preparation hydrolyzed both the 1- and 2-ester bonds of phosphatidylcholine. When degumming of soybean, rapeseed, and peanut oil was performed with this enzyme preparation, oils with phosphorous contents lower than 5mg/kg were obtained after 5h of enzyme treatment at 40°C. The enzyme preparation did not show lipase activity, thus free fatty acids were only generated from the phospholipids. Therefore, this novel phospholipase B is potentially useful for the refining of high-quality oils with attractive yields. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 102
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 63560354
- Full Text :
- https://doi.org/10.1016/j.biortech.2011.05.050