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High shear flow induces migration of adherent human platelets.

Authors :
Kraemer, Bjoern F.
Schmidt, Christine
Urban, Benjamin
Bigalke, Boris
Schwanitz, Laura
Koch, Miriam
Seizer, Peter
Schaller, Martin
Gawaz, Meinrad
Lindemann, Stephan
Source :
Platelets. Sep2011, Vol. 22 Issue 6, p415-421. 7p. 1 Color Photograph, 3 Graphs.
Publication Year :
2011

Abstract

Shear forces are generated in all parts of the vascular system and contribute directly and indirectly to vascular disease progression. Endothelial cells are able to adapt to flow conditions, and are known to polarize and migrate in response to shear forces. Platelets exposed to shear stress are activated and release bioactive molecules from their alpha granules. So far, platelets have been considered to be static cells that do not leave the site of tight adhesion. However, we have recently been able to demonstrate the capacity of platelets to migrate in response to stromal derived factor-1 (SDF-1). In this project, we have demonstrated that platelets accumulate in areas with a high concentration of SDF-1 under flow conditions and respond to high shear stress by cellular polarization, cytoskeletal reorganisation, and flow-directed migration. In this context, we have shown increased Wiskott-Aldrich Syndrome protein (WASP) phosphorylation and intracellular redistribution of focal adhesion kinase (FAK) under high-shear stress conditions. The effect of flow-induced platelet migration has not previously been recognized and offers a new role for platelets as mobile cells. Their migratory potential may enable platelets to cover intimal lesions and contribute to vascular repair. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09537104
Volume :
22
Issue :
6
Database :
Academic Search Index
Journal :
Platelets
Publication Type :
Academic Journal
Accession number :
63701805
Full Text :
https://doi.org/10.3109/09537104.2011.556277