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TRPV4-Mediated Calcium Influx into Human Bronchial Epithelia upon Exposure to Diesel Exhaust Particles.
- Source :
-
Environmental Health Perspectives . Jun2011, Vol. 119 Issue 6, p784-793. 10p. - Publication Year :
- 2011
-
Abstract
- Background: Human respiratory epithelia function in airway mucociliary clearance and barrier function and have recently been implicated in sensory functions. oBjective: We investigated a link between chronic obstructive pulmonary disease (COPD) patho­genesis and molecular mechanisms underlying Ca2+ influx into human airway epithelia elicited by diesel exhaust particles (DEP). Methods and results: Using primary cultures of human respiratory epithelial (HRE) cells, we determined that these cells possess proteolytic signaling machinery, whereby proteinase-activated receptor-2 (PAR-2) activates Ca2+-permeable TRPV4, which leads to activation of human respira­tory disease-enhancing matrix metalloproteinase-1 (MMP-1), a signaling cascade initiated by diesel exhaust particles (DEP), a globally relevant air pollutant. Moreover, we observed ciliary expression of PAR-2, TRPV4, and phospholipase-C?3 in human airway epithelia and their DEP-enhanced protein-protein complex formation. We also found that the chronic obstructive pulmonary disease (COPD)-predisposing TRPV4P19S variant enhances Ca2+ influx and MMP 1 activation, providing mechanistic linkage between man-made air pollution and human airway disease. conclusion: DEP evoked protracted Ca2+ influx via TRPV4, enhanced by the COPD-predisposing human genetic polymorphism TRPV4P19S. This mechanism reprograms maladaptive inflammatory and extracellular-matrix-remodeling responses in human airways. The novel concept of air pollu­tion-responsive ciliary signal transduction from PAR-2 to TRPV4 in human respiratory epithelia will accelerate rationally targeted therapies, possibly via the inhalatory route. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00916765
- Volume :
- 119
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Environmental Health Perspectives
- Publication Type :
- Academic Journal
- Accession number :
- 63933601
- Full Text :
- https://doi.org/10.1289/ehp.1002807