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Expression of CD34 and CD7 on human T-cell acute lymphoblastic leukemia discriminates functionally heterogeneous cell populations.
- Source :
-
Leukemia (08876924) . Aug2011, Vol. 25 Issue 8, p1249-1258. 10p. 5 Graphs. - Publication Year :
- 2011
-
Abstract
- Leukemia-initiating/repopulating cells (LICs), also named leukemic stem cells, are responsible for propagating human acute leukemia. Although they have been characterized in various leukemias, their role in T-cell acute lymphoblastic leukemia (T-ALL) is unclear. To identify and characterize LICs in T-ALL (T-LIC), we fractionated peripheral blood cell populations from patient samples by flow cytometry into three cell fractions by using two markers: CD34 (a marker of immature cells and LICs) and CD7 (a marker of early T-cell differentiation). We tested these populations in both in vitro culture assays and in vivo for growth and leukemia development in immune-deficient mice. We found LIC activity in CD7+ cells only as CD34+CD7− cells contained normal human progenitors and hematopoietic stem cells that differentiated into T, B lymphoid and myeloid cells. In contrast, CD34+CD7+ cells were enriched in LICs, when compared with CD34−CD7+ cells. These CD34+CD7+ cells also proliferated more upon NOTCH activation than CD34−CD7+ cells and were sensitive to dexamethasone and NOTCH inhibitors. These data show that CD34 and CD7 expression in human T-ALL samples help in discriminating heterogeneous cell populations endowed with different LIC activity, proliferation capacity and responses to drugs. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LYMPHOBLASTIC leukemia
*CELL populations
*STEM cells
*T-cell lymphoma
*CORD blood
Subjects
Details
- Language :
- English
- ISSN :
- 08876924
- Volume :
- 25
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Leukemia (08876924)
- Publication Type :
- Academic Journal
- Accession number :
- 64003157
- Full Text :
- https://doi.org/10.1038/leu.2011.93