Dense aqueous colloidal gold nanoparticles prepared from highly concentrated precursor solution

Abstract: Gold nanoparticles were fabricated by reduction of highly concentrated Au(III) ions (200mM) with casein proteins from milk. The gold nanoparticles were converted to nanoparticle-powders after washing and subsequent vacuum drying without aggregation. The nanoparticle-powders completely re-dispersed in aqueous solution, and stable colloidal gold nanoparticles were obtained. UV–vis extinction spectra and dynamic light scattering (DLS) measurements revealed that large assemblies (size, ca. 3μm) and subaggregates (size, <0.5μm) composed of gold nanoparticle–casein protein chain–Au(III) ion were dynamically formed and disintegrated over the course of the growth of the gold nanoparticles. Fourier transform infrared (FT-IR) spectra indicated conformational changes of casein proteins induced by the interaction of casein protein–Au(III) ion and -gold nanoparticle. Finally, rapid, one-pot, and highly concentrated synthetic procedures of gold and silver nanoparticle powders protected by casein (mean diameters below 10nm) were successfully developed using 3-amino-1-propanol aqueous solutions as reaction media. Dense colloidal gold (40gL−1) and silver (22gL−1) nanoparticle aqueous solutions were obtained by re-dispersing the metal nanoparticle powders. [Copyright &y& Elsevier]