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Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections.
- Source :
-
FEMS Immunology & Medical Microbiology . Oct2011, Vol. 63 Issue 1, p10-15. 6p. 3 Charts, 2 Graphs. - Publication Year :
- 2011
-
Abstract
- Biofilms play a pivotal role in medical device-related infections. However, epidemiological analysis of biofilm formation and genotyping among clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients with orthopaedic infections has rarely been reported. A total of 168 MRSA strains were examined: 23 strains from patients with device-related infection (the device group); 55 from patients with device-non-related infection (the nondevice group); and 90 from asymptomatic nasal carriers (the colonization group). Pulsed-field gel electrophoresis analysis and five genotyping methods including agr typing were performed. Biofilm formation was quantified using a microtitre plate assay. The device group had a significantly higher incidence of agr-2 than the colonization group (78.3% vs. 34.4%, P=0.001). The biofilm index of the agr-2 (0.523 ± 0.572) strains was significantly higher than those of agr-1 (0.260 ± 0.418, P<0.0001) and agr-3 (0.379 ± 0.557, P=0.045). The prevalence of strong biofilm formers in the device group (43.5%) was significantly higher than that in the nondevice group (12.7%, P=0.003) and the colonization group (20.0%, P=0.020). agr-2 MRSA strains may be more likely to cause orthopaedic device infection because of their strong biofilm formation ability. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09288244
- Volume :
- 63
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- FEMS Immunology & Medical Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 65278798
- Full Text :
- https://doi.org/10.1111/j.1574-695X.2011.00821.x