Back to Search
Start Over
Fate of Escherichia coli O157:H7 in field-inoculated lettuce
- Source :
-
Food Microbiology . Dec2011, Vol. 28 Issue 8, p1417-1425. 9p. - Publication Year :
- 2011
-
Abstract
- Abstract: Impact of drip and overhead sprinkler irrigation on the persistence of attenuated Escherichia coli O157:H7 in the lettuce phyllosphere was investigated using a split-plot design in four field trials conducted in the Salinas Valley, California, between summer 2007 and fall 2009. Rifampicin-resistant attenuated E. coli O157:H7 ATCC 700728 (BLS1) was inoculated onto the soil beds after seeding with a backpack sprayer or onto 2- or 4-week-old lettuce plant foliage with a spray bottle at a level of 7 log CFU ml−1. When E. coli O157:H7 was inoculated onto 2-week-old plants, the organism was recovered by enrichment in 1 of 120 or 0 of 240 plants at 21 or 28 days post-inoculation, respectively. For the four trials where inoculum was applied to 4-week-old plants, the population size of E. coli O157:H7 declined rapidly and by day 7, counts were near or below the limit of detection (10 cells per plant) for 82% or more of the samples. However, in 3 out 4 field trials E. coli O157:H7 was still detected in lettuce plants by enrichment 4-weeks post-inoculation. Neither drip nor overhead sprinkler irrigation consistently influenced the survival of E. coli O157:H7 on lettuce. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 07400020
- Volume :
- 28
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Food Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 65514594
- Full Text :
- https://doi.org/10.1016/j.fm.2011.02.001