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Levobupivacaine-induced contraction of isolated rat aorta is calcium dependent.

Authors :
Baik, Ji Seok
Sohn, Ju-Tae
Ok, Seong-Ho
Kim, Jae-Gak
Sung, Hui-Jin
Park, Sang-Seung
Park, Jae-Yong
Hwang, Eun Mi
Chung, Young-Kyun
Source :
Canadian Journal of Physiology & Pharmacology. Jul2011, Vol. 89 Issue 7, p467-476. 10p.
Publication Year :
2011

Abstract

Levobupivacaine is a long-acting local anesthetic that intrinsically produces vasoconstriction in isolated vessels. The goals of this study were to investigate the calcium-dependent mechanism underlying levobupivacaine-induced contraction of isolated rat aorta in vitro and to elucidate the pathway responsible for the endothelium-dependent attenuation of levobupivacaine-induced contraction. Isolated rat aortic rings were suspended to record isometric tension. Cumulative levobupivacaine concentration-response curves were generated in either the presence or absence of the antagonists verapamil, nifedipine, SKF-96365, 2-aminoethoxydiphenylborate, Gd3+, N W-nitro- l-arginine methyl ester (L-NAME), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), and methylene blue, either alone or in combination. Verapamil, nifedipine, SKF-96365, 2-aminoethoxydiphenylborate, low calcium concentrations, and calcium-free Krebs solution attenuated levobupivacaine-induced contraction. Gd3+ had no effect on levobupivacaine-induced contraction. Levobupivacaine increased intracellular calcium levels in vascular smooth muscle cells. L-NAME, ODQ, and methylene blue increased levobupivacaine-induced contraction in endothelium-intact aorta. SKF-96365 attenuated calcium-induced contraction in a previously calcium-free isotonic depolarizing solution containing 100 mmol/L KCl. Levobupivacaine-induced contraction of rat aortic smooth muscle is mediated primarily by calcium influx from the extracellular space mainly via voltage-operated calcium channels and, in part, by inositol 1,4,5-trisphosphate receptor-mediated release of calcium from the sarcoplasmic reticulum. The nitric oxide - cyclic guanosine monophosphate pathway is involved in the endothelium-dependent attenuation of levobupivacaine-induced contraction. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00084212
Volume :
89
Issue :
7
Database :
Academic Search Index
Journal :
Canadian Journal of Physiology & Pharmacology
Publication Type :
Academic Journal
Accession number :
65536330
Full Text :
https://doi.org/10.1139/y11-046