Hydrogen peroxide–sodium hydrosulfite chemiluminescence system combined with high-performance liquid chromatography for determination of 1-hydroxypyrene in airborne particulates

Abstract: In this research, a highly sensitive chemiluminescence method based on a sodium hydrosulfite (NaHSO3)–hydrogen peroxide (H2O2) reaction for the determination of 1-hydroxypyrene (1-OHP) was developed. The response of this system was linear in the range from 0.5 to 50pmol (R 2 =0.9983). The limit of detection for 1-OHP was 100fmol (S/N =3). 1-OHP in airborne particulates was well separated from interfering compounds using an ODS column with 75% methanol as the mobile phase in isocratic mode. The proposed method was successfully applied to determine the 1-OHP in airborne particulates collected in Kanazawa, Japan. The average concentration of 1-OHP in the atmosphere was 2.0pg/m3 (9.2fmol/m3). [Copyright &y& Elsevier]