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Brn-3b inhibits generation of amacrine cells by binding to and negatively regulating DLX1/2 in developing retina
- Source :
-
Neuroscience . Nov2011, Vol. 195, p9-20. 12p. - Publication Year :
- 2011
-
Abstract
- Abstract: During retinogenesis, the basic helix-loop-helix proneural gene math5 (atoh7) initiates the generation of the first-born neurons, retinal ganglion cells (RGCs), by activating a network of RGC transcription factors, including Brn-3b (POU4F2). Herein, we show that the expression of DLX1 and DLX2 is significantly down-regulated in math5-null retina but is markedly increased in Brn-3b-null retina. Interestingly, Brn-3b interacts with DLX1 through its homeodomain, and this interaction represses DLX1 activity. Retrovirus-mediated mis-expression of DLX1 or DLX2 dramatically increases the number of amacrine/bipolar cells and concurrently reduces rod photoreceptors. Conversely, combined ectopic expression of Brn-3b with DLX1 or DLX2 promotes the production of RGCs and inhibits amacrine cell differentiation. Thus, DLX1/2 play an essential role in cell fate selection between amacrine and RGCs. Brn-3b suppresses the role of DLX1/2 through physical interaction and biases the competent precursors toward RGC fates. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 03064522
- Volume :
- 195
- Database :
- Academic Search Index
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 66491939
- Full Text :
- https://doi.org/10.1016/j.neuroscience.2011.08.015