Structural insight into the stereoselective production of PGF2α by Old Yellow Enzyme from Trypanosoma cruzi.

Old yellow enzyme (OYE) is an NADPH oxidoreductase capable of reducing a variety of compounds. It contains flavin mononucleotide (FMN) as a prosthetic group. A ternary complex structure of OYE from Trypanosoma cruzi (TcOYE) with FMN and one of the substrates, p-hydroxybenzaldehyde, shows a striking movement around the active site upon binding of the substrate. From a structural comparison of other OYE complexed with 12-oxophytodienoate, we have constructed a complex structure with another substrate, prostaglandin H2 (PGH2), to provide a proposed stereoselective reaction mechanism for the reduction of PGH2 to prostaglandin F2α by TcOYE. [ABSTRACT FROM PUBLISHER]