Back to Search
Start Over
Culture of embryos and shoot tips for chromosome doubling in Lolium perenne and sterile hybrids between Lolium and Festuca.
- Source :
-
Plant Breeding . Apr2000, Vol. 119 Issue 2, p185-187. 0p. - Publication Year :
- 2000
-
Abstract
- An improved method is reported for polyploid induction in Lolium (ryegrass), and in sterile F1 hybrids between Lolium and Festuca (fescue). Two factors greatly increased the survival rate of colchicine-treated embryos of Lolium perenne (perennial ryegrass) germinated and cultured in vitro: (1) a high concentration of sucrose (100 g/l) in the germination medium and (2), maintenance at a low temperature of 10°C for 2 weeks after treatment. The maximum success rate for chromosome doubling among survivors of perennial ryegrass was 79.1%, and for L. perenne × Festuca arundinacea F1 hybrid embryos it exceeded 40%. The same doubling treatment also works with shoot tip culture in non-flowering genotypes obtained by anther culture of L. multiflorum × F. arundinacea hybrids. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RYEGRASSES
*FESCUE
Subjects
Details
- Language :
- English
- ISSN :
- 01799541
- Volume :
- 119
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Plant Breeding
- Publication Type :
- Academic Journal
- Accession number :
- 6703329
- Full Text :
- https://doi.org/10.1046/j.1439-0523.2000.00484.x