Back to Search
Start Over
The effects of CD59 gene as a target gene on breast cancer cells
- Source :
-
Cellular Immunology . Dec2011, Vol. 272 Issue 1, p61-70. 10p. - Publication Year :
- 2011
-
Abstract
- Abstract: The retroviral-vector-targeted CD59 gene (pSUPER-siCD59) was constructed and transfected into breast cells (MCF-7). The results demonstrated that the retroviral vector-mediated RNAi successfully suppressed human CD59 gene. The expression of CD59 decreased at both mRNA and protein levels. Knockdown of CD59 abrogated its protective effect on complement-mediated cytolysis. Fas and caspase-3 were remarkably upregulated, which induced apoptosis and tumor growth suppression in MCF-7 cells. In addition, overexpression of CD59 promoted the proliferation of MCF-7 cells and inhibited anti-apoptotic Bcl-2 expression. In conclusion, CD59 may be a promising target in the gene therapy of breast cancer. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00088749
- Volume :
- 272
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cellular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 67207703
- Full Text :
- https://doi.org/10.1016/j.cellimm.2011.09.006