Selection of functional consortium for crude oil-contaminated soil remediation

Abstract: Microorganisms with high oil-degrading performance are essential for bioremediation of soil contaminated with crude oil. A positive end dilution method was employed for the selection of crude oil-degrading functional consortium from contaminated soil. The selected consortium was consisted of Rhizobiales sp., Pseudomonas sp., Brucella sp., Bacillus sp., Rhodococcus sp., Microbacterium sp. and Roseomonas sp. and removed nearly 52.1% of crude oil at initial concentration of 10,000 mg l−1 at 30 °C within 7 days, with removal of aliphatic hydrocarbons by 71.4% and aromatic hydrocarbons by 36.0%, respectively. The effectiveness of the consortium for bioaugmentation was confirmed with microcosm test by contaminated soil (1.0 kg) from Karemary Oilfield, China. The removal efficiency of crude oil was enhanced to >50% in microcosms with the consortium compared with 8–13% or lower in controls over a 60 day period. The crude oil removal reaction was probably first order reaction and the rate was greatly enhanced by bioaugmentation. Supplementation of nitrogen and phosphate sources had limited effect on the oil removal in the tested soil. [Copyright &y& Elsevier]