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Pharmacological inhibition of AKT sensitizes MCF-7 human breast cancer-initiating cells to radiation.
- Source :
-
Cellular Oncology (2211-3428) . Oct2011, Vol. 34 Issue 5, p451-456. 6p. - Publication Year :
- 2011
-
Abstract
- Background: Caner-initiating cells (CICs or cancer stem cells) have been shown both experimentally and clinically to be resistant to radiation. The mechanism underlying radioresistance remains unclear. Methods: In the present study, we screened 51 genes which are potentially important in mediating radioresistance of breast CICs. Results: The expression of AKT1 and AKT2 at protein and mRNA levels was dramatically increased among the screened genes by 8 Gy radiation treatment in MCF-7 mammosphere cells (predominantly CD24/CD44 CICs), but not in the bulk population of MCF-7 cells (predominantly CD24/CD44). Using apoptosis and clonogenic survival assays, we found pharmacological inhibition of AKT with selective inhibitors of AKT sensitized MCF-7 mammosphere cells, but not MCF-7 monolayer cells to radiation. Conclusion: The present findings suggest that treatment with AKT inhibitors prior to ionizing radiation treatment may be a potential benefit to patients with breast cancer, in particular to eradiate breast CICs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22113428
- Volume :
- 34
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Cellular Oncology (2211-3428)
- Publication Type :
- Academic Journal
- Accession number :
- 67281091
- Full Text :
- https://doi.org/10.1007/s13402-011-0020-1