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LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis
- Source :
-
Bioresource Technology . Jan2012, Vol. 103 Issue 1, p266-272. 7p. - Publication Year :
- 2012
-
Abstract
- Abstract: A unique method that applied a multilayer-immobilization strategy was developed to prepare nanofibrous enzymes for biosynthesis. LiCl co-electrospun with polyurethane nanofibers enabled strong physical adsorption of bovine serum albumin (BSA), forming the first layer of protein on the nanofibers; lipase AK was subsequently crosslinked to BSA as an outer layer of enzyme. The content of LiCl in nanofibers was found to be a sensitive factor affecting the activity and stability of the immobilized lipase. For biodiesel synthesis from soybean oil and methanol in isooctane, the reaction rate catalyzed by nanofibrious lipase carrying 5wt% LiCl was 6.6-fold higher than fibers without LiCl, with a conversion of 91% was achieved within 2h. LiCl also induced much improved enzyme stability. The nanofibrous lipase with 5% LiCl could be repeatedly used for 42 cycles without apparent activity loss, while the immobilized lipase without LiCl lost over 90% activity within 13 reuse cycles. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 103
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 67382471
- Full Text :
- https://doi.org/10.1016/j.biortech.2011.09.089