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Brush and Spray: A High-Throughput Systemic Acquired Resistance Assay Suitable for Large-Scale Genetic Screening.
- Source :
-
Plant Physiology . Nov2011, Vol. 157 Issue 3, p973-980. 8p. - Publication Year :
- 2011
-
Abstract
- Systemic acquired resistance (SAR) is a defense mechanism induced in the distal parts of plants after primary infection. It confers long-lasting protection against a broad spectrum of microbial pathogens. Lack of high-throughput assays has hampered the forward genetic analysis of SAR. Here, we report the development of an easy and efficient assay for SAR and its application in a forward genetic screen for SAR-deficient mutants in Arabidopsis (Arabidopsis thaliana). Using the new assay for SAR, we identified six flavin-dependent monooxygenasel, four AGD2-Iike defense response protein1, three salicylic acid induction-deficient2, one phytoalexin deficient4, and one avrPphB-susceptible3 alleles as well as a gain-of-function mutant of CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR3 designated camta3-3D. Like transgenic plants overexpressing CAMTA3, camta3-3D mutant plants exhibit compromised SAR and enhanced susceptibility to virulent pathogens, suggesting that CAMTA3 is a critical regulator of both basal resistance and SAR. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00320889
- Volume :
- 157
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Plant Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 67618662
- Full Text :
- https://doi.org/10.1104/pp.111.182089