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Amplified Analysis of DNA by the Autonomous Assembly of Polymers Consisting of DNAzyme Wires.
- Source :
-
Journal of the American Chemical Society . 11/2/2011, Vol. 133 Issue 43, p17149-17151. 3p. - Publication Year :
- 2011
-
Abstract
- A systematic study of the amplified optical detection of DNA by Mg2+-dependent DNAzyme subunits is described. The use of two DNAzyme subunits and the respective fluorophore/quencher-modified substrate allows the detection of the target DNA with a sensitivity corresponding to 1 × 10-9 M. The use of two functional hairpin structures that include the DNAzyme subunits in a caged, inactive configuration leads, in the presence of the target DNA, to the opening of one of the hairpins and to the activation of an autonomous cross-opening process of the two hairpins, which affords polymer DNA wires consisting of the Mg2+-dependent DNAzyme subunits. This amplification paradigm leads to the analysis of the target DNA with a sensitivity corresponding to 1 × 10-14 M. The amplification mixture composed of the two hairpins can be implemented as a versatile sensing platform for analyzing any gene in the presence of the appropriate hairpin probe. This is exemplified with the detection of the BRCA1 oncogene. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA analysis
*POLYMERS
*ONCOGENES
*GENE amplification
*SPECTRAL sensitivity
Subjects
Details
- Language :
- English
- ISSN :
- 00027863
- Volume :
- 133
- Issue :
- 43
- Database :
- Academic Search Index
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 67634754
- Full Text :
- https://doi.org/10.1021/ja2076789