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Macrophage Migration Inhibitory Factor Isolated from a Parasite Inhibited Th2 Cytokine Production in PBMCs of Atopic Asthma Patients.
- Source :
-
Journal of Asthma . Feb2012, Vol. 49 Issue 1, p10-15. 6p. - Publication Year :
- 2012
-
Abstract
- Background. In a previous study, we demonstrated that the human macrophage migration inhibitory factor (MIF)-like protein (As-MIF) isolated from helminths could inhibit allergic airway inflammation via the recruitment of CD4+CD25+Foxp3+ T cells. Objective. To evaluate the clinical importance of As-MIF as an antiasthma drug, we evaluated immune responses after recombinant As-MIF (rAs-MIF) treatment in peripheral blood mononuclear cell (PBMC) cultures. Methods. PBMC was isolated from 10 patients with atopic asthma, 8 patients with nonatopic asthma, and 12 nonatopic healthy subjects, and various concentrations of rAs-MIF were transferred into the PBMC culture medium. After 3 days, we measured the levels of T helper 2 and T helper 1 cytokines via ELISA. Results. In atopic asthma, IL-4 and IL-5 production was significantly reduced in the PBMC cultures after rAs-MIF treatment. These inhibitory effects were not observed in the nonatopic asthma group. By way of contrast, IL-10 production in the PMBC cultures was significantly increased after rAs-MIF treatment in all experimental groups. Conclusion. The results of this study are similar to those previously reported in a mouse study, suggesting that As-MIF might be a candidate for the specific treatment of asthma. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02770903
- Volume :
- 49
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Asthma
- Publication Type :
- Academic Journal
- Accession number :
- 70248840
- Full Text :
- https://doi.org/10.3109/02770903.2011.637593