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Effect of glucagon-like peptide-1 gene expression on graft function in mouse islet transplantation.
- Source :
-
Transplant International . Feb2012, Vol. 25 Issue 2, p242-249. 8p. - Publication Year :
- 2012
-
Abstract
- Summary This study investigated the effect of local glucagon-like peptide-1 (GLP-1) production within mouse islets on cytoprotection in vitro and in vivo by gene transfer of GLP-1. Transduction of recombinant adenovirus vector expressing GLP-1 (rAd-GLP-1) induced a significant increase in bioactive GLP-1 in the mouse islet culture, whereas transduction with adenovirus vector expressing β-galactosidase (rAd-LacZ), as a control, had no effect on GLP-1 secretion. Islets transduced with rAd-GLP-1 were protected from H2O2-induced cell damage in vitro. In addition, glucose-stimulated insulin secretion was significantly increased in rAd-GLP-1-transduced islets. When transplanted under the kidney capsule of diabetic syngeneic mice, islet grafts retrieved 4 or 7 days after transplantation revealed that the rAd-GLP-1-transduced group had significantly more Ki67-positive cells as compared with the rAd-LacZ-transduced group. Regarding blood glucose control, diabetic mice transplanted with a marginal mass of rAd-GLP-1-transduced islets became normoglycemic more rapidly and 78% of the recipients were normoglycemic at 35 days post-transplant, whereas only 48% of the mice transplanted with rAd-LacZ-transduced islets were normoglycemic ( P < 0.05). In conclusion, delivery of the GLP-1 gene to islets enhanced islet cell survival during the early post-transplant period, and preserved islet mass and functions over time in the transplants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09340874
- Volume :
- 25
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Transplant International
- Publication Type :
- Academic Journal
- Accession number :
- 70330180
- Full Text :
- https://doi.org/10.1111/j.1432-2277.2011.01394.x