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MALDI-TOF MS improves routine identification of non-fermenting Gram negative isolates from cystic fibrosis patients
- Source :
-
Journal of Cystic Fibrosis . Jan2012, Vol. 11 Issue 1, p59-62. 4p. - Publication Year :
- 2012
-
Abstract
- Abstract: Identification of non-fermenting Gram-negative bacteria (NFGNB) from cystic fibrosis (CF) patients is often limited. A collection of stored NFGNB isolates (n=182) recovered from CF patients over a 15year period was examined. The routinely reported identification during this period was compared with that obtained by MALDI-TOF MS. Isolates giving discrepant identification at the genus level were further analyzed by 16S rDNA sequencing. The MALDI-TOF MS system identified 94% of the isolates, including Burkholderia cepacia and Pandoraea spp. isolates, the latter previously misidentified as other NFGNB by conventional microbiological methods. Lack of identification by MALDI-TOF MS was associated with the absence of entries in the database. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 15691993
- Volume :
- 11
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Cystic Fibrosis
- Publication Type :
- Academic Journal
- Accession number :
- 70389741
- Full Text :
- https://doi.org/10.1016/j.jcf.2011.09.001