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Modified apple polysaccharide prevents against tumorigenesis in a mouse model of colitis-associated colon cancer: role of galectin-3 and apoptosis in cancer prevention.
- Source :
-
European Journal of Nutrition . Feb2012, Vol. 51 Issue 1, p107-117. 11p. 2 Color Photographs, 1 Chart, 3 Graphs. - Publication Year :
- 2012
-
Abstract
- Background: Colorectal cancer (CRC) is one of the most common and preventable cancers. Regular consumption of apples is conducive to reduction in CRC risk. Aim of the study: To evaluate effects of modified apple polysaccharide (MAP) on tumorigenesis in a mouse model of colitis-associated colon cancer. Methods: One hundred male ICR mice were administered with 1, 2-dimethyl-hydrazine (DMH) and dextran sodium sulfate (DSS). Forty mice were given no further treatment, the rest were fed basal diet blended with three different doses of MAP; 2.5, 5, and 10% (20 mice in each group). Results: MAP significantly protected ICR mice against DMH/DSS-induced tumorigenesis. The incidence of tumor development was 90% (18/20) in the mice treated with DMH/DSS, but that was reduced to 25% (5/20), 15% (3/20), and 5% (1/20), respectively, in the mice treated with basal diets plus 2.5, 5, and 10% of MAP. Study of apoptosis of colonic epithelial cells revealed that MAP moderately increased apoptosis, suggesting that the anti-tumor potency of MAP was probably attributed to its ability to induce apoptosis. Western blot analysis demonstrated that carbohydrate-binding protein galectin-3 changed in both the nucleus and the cytoplasm during the process from colitis to colon cancer in the model. And MAP could inhibit the binding of galectin-3 to its ligand: this is, at least in part, the possible mechanism of MAP by enhancing apoptosis and preventing tumorigenesis. Conclusions: These data suggest that MAP has a potential role in clinical prevention and treatment for colon cancer. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENZYME analysis
*COLON tumor prevention
*ANALYSIS of variance
*ANIMAL experimentation
*ANTINEOPLASTIC agents
*APPLES
*BIOLOGICAL assay
*BIOPHYSICS
*CHI-squared test
*HISTOLOGICAL techniques
*IMMUNOHISTOCHEMISTRY
*RESEARCH methodology
*MICE
*POLYSACCHARIDES
*RESEARCH funding
*T-test (Statistics)
*WESTERN immunoblotting
*PLANT extracts
*STATISTICAL significance
*DESCRIPTIVE statistics
*PHARMACODYNAMICS
TUMOR prevention
RECTUM tumors
Subjects
Details
- Language :
- English
- ISSN :
- 14366207
- Volume :
- 51
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 70531098
- Full Text :
- https://doi.org/10.1007/s00394-011-0194-3