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Ganoderic acid DM, a natural triterpenoid, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells
- Source :
-
Fitoterapia . Mar2012, Vol. 83 Issue 2, p408-414. 7p. - Publication Year :
- 2012
-
Abstract
- Abstract: Ganoderic acid DM (GADM) is a triterpenoid isolated from Ganoderma lucidum, a well-known edible medicinal mushroom. In the present study, we found that GADM effectively inhibited cell proliferation and colony formation in MCF-7 human breast cancer cells, which was much stronger than that of MDA-MB-231 breast cancer cells. GADM both concentration- and time-dependently mediated G1 cell cycle arrest and significantly decreased the protein level of CDK2, CDK6, cycle D1, p-Rb and c-Myc in MCF-7 cells. Moreover, GADM obviously induced DNA fragmentation and cleavage of PARP which are the characteristics of apoptosis and decreased the mitochondrial membrane potential in MCF-7 cells. Besides, we also showed that GADM elicited DNA damage as measured by comet assay which is a sensitive method for DNA damage detection. γ-H2AX, a marker of DNA damage, was also slightly up-regulated after treated with GADM for 6h, suggesting that the G1 cell cycle arrest and apoptosis induced by GADM may be partially resulted from GADM-induced DNA damage. These results have advanced our current understandings of the anti-cancer mechanisms of GADM. [Copyright &y& Elsevier]
- Subjects :
- *ALTERNATIVE medicine
*ANTINEOPLASTIC agents
*APOPTOSIS
*BIOLOGICAL assay
*BIOLOGICAL models
*BIOLOGICAL transport
*BIOPHYSICS
*DOSE-effect relationship in pharmacology
*RESEARCH methodology
*MITOCHONDRIA
*EDIBLE mushrooms
*PLANT extracts
*STATISTICAL significance
*DESCRIPTIVE statistics
*PHARMACODYNAMICS
BREAST tumor prevention
Subjects
Details
- Language :
- English
- ISSN :
- 0367326X
- Volume :
- 83
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Fitoterapia
- Publication Type :
- Academic Journal
- Accession number :
- 70949997
- Full Text :
- https://doi.org/10.1016/j.fitote.2011.12.004