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Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide
- Source :
-
Journal of Bioscience & Bioengineering . Feb2012, Vol. 113 Issue 2, p160-165. 6p. - Publication Year :
- 2012
-
Abstract
- Abstract: We report that several tryptophan-rich peptides exhibit an affinity for a hydrophobic ionic liquid (IL) (1-ethyl-3-methylimidazolium bis-trifluoromethanesulfonyl imide), and that green fluorescent protein (GFP) fused to a peptides, “SSSWWSWWWW” (SW1) or “SWWWWSWWWW” (SW2), containing serine (S) and tryptophan (W) at the C terminus localized at the IL/water interface. While GFPs without W-rich peptide distributed only in water phase, SW1- and SW2-GFPs were accumulated at the interface. The localization of SW1-GFP showed biphasic behavior, and most distinctive localization was observed at 7.1μM. The localization of SW2-GFP presumably occurred at largely lower concentration (≤0.5μM) than that of SW1-GFP, which difference was due to the higher hydrophobicity of SW2 peptide. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 13891723
- Volume :
- 113
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Bioscience & Bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 71696948
- Full Text :
- https://doi.org/10.1016/j.jbiosc.2011.09.016