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DNA Interaction Studies of a New Platinum(II) Complex Containing Different Aromatic Dinitrogen Ligands.
- Source :
-
Bioinorganic Chemistry & Applications . 2011, Vol. 2011, Special section p1-8. 8p. - Publication Year :
- 2011
-
Abstract
- A new mononuclear Pt(II) complex, [Pt(DMP)(DIP)]Cl2.H2O, in which DMP is 4,4-dimethyl-2,2-bipyridine and DIP is 4,7-diphenyl-1,10-phenantroline, has been synthesized and characterized by physicochemical and spectroscopic methods. The binding interaction of this complex with calf thymus DNA (CT-DNA) was investigated using fluorimetry, spectrophotometry, circular dichroism, viscosimetry and cyclic voltametry (CV). UV-VIS spectrum showed 4 nm bathochromic shift of the absorption band at 280 nm along with significant hypochromicity for the absorption band of the complex. The intrnisic binding constant (Kb = 2 x 104 M-1) is more in keeping with intercalators and suggests this binding mode. The viscosity measurements showed that the complex- DNA interaction can be hydrophobic and confirm intercalation. Moreover, the complex induced detectable changes in the CD spectrum of CT-DNA. The fluorescence studies revealed that the probable quenching mechanism of fluorescence of the complex by CT-DNA is static quenching. The thermodynamic parameters (ΔH > 0 and ΔS > 0) showed that main interaction with hydrogenic forces occurred that is intercalation mode. Also, CV results confirm this mode because, with increasing the CT-DNA concentration, shift to higher potential was observed. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15653633
- Volume :
- 2011
- Database :
- Academic Search Index
- Journal :
- Bioinorganic Chemistry & Applications
- Publication Type :
- Academic Journal
- Accession number :
- 71894587
- Full Text :
- https://doi.org/10.1155/2011/429241